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KMID : 0357819950190010054
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Korean Journal of Legal Medicine
1995 Volume.19 No. 1 p.54 ~ p.0
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Allele Frequency and Genotype Distribution of STR TC-11 Locus in Korean
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최찬 이정빈
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Abstract
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DNA was obtained from placental tissue and peripheral blood of 200 unrelated Korean in order to analyze the applicability of genetic polymorphism of human tyrosine hydroxylase gene(TC-11) STR(short tandem repeat) locust to forensic medicine in
the
field
of individual identification and paternity test. After amplification using two primers specific to the TC-11 locus, the products were separated separated by polyacrylamide gel electrophoresis, so called Amp-FLP(Amplification Fragment Length
Polymorphism) procedure followed by silver staining. Seven alleles were deteced, but allele 10 and allele 8 constituted the majority and the number of core unit repetition ranged from 7 to 13. The heterozygosity was 69.5% and 17 different
genotypes
were
revealed. One of the alleles was sequenced for the confirmation of exact size and repeatition unit. In 164 gametes of 64 family whose parent-child relationship were confirmed through other studies, Mendelian inheritance was well presented in
every
case,
and no mutation was observed. But in practical work, the application could be limited in case of allele 10 and allele 8 due to their high frequencies.
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KEYWORD
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